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異丙基 β-D -硫代半乳糖吡喃糖苷 溶液
Isopropyl β-D -thiogalactopyranoside solution
| 產品編號： | 3101693 |
| 產品類別： | 非離子去污劑 |
| 純度規格： | ReadyMade IPTG solution for Blue-white screening |
| 包裝規格： | 5 ML |
| 市場價： | 1200元 |
| 優惠價： | 立即咨詢 |
367-93-1
異丙基 β-
Isopropyl β-
IPTG solution
IPTG 溶液
結構式
基本信息
| Empirical Formula【經驗(實驗)分子式】 | C9H18O5S |
| Molecular weight | 238.30 |
| Beilstein | 4631 |
| MDL number | MFCD00063273 |
| PubChem Substance ID【PubChem化學物質編號】 | 24895928 |
| NACRES | NA.52 |
| General description【一般描述】 | Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 ℃), thus avoiding freeze-thaw cycles and providing a user-friendly solution. |
| Application【應用】 | Isopropyl β-
|
| Biochem/physiol Actions【生化/生理作用】 | Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment. |
| Components【組分】 | IPTG Solution is composed of 200 mM IPTG in molecular biology grade water. |
| Principle【原理】 | Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. |
產品性質
| Quality Level【質量水平】 | 200 |
| grade【等級】 | for molecular biology |
| description【描述】 | non-ionic |
| form【形式】 | liquid |
| shipped in【運輸】 | dry ice |
| storage temp.【儲存溫度】 | −20℃ |
| SMILES string | CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O |
| InChI | 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1 |
| InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
安全信息
| Storage Class Code【儲存分類代碼】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| Personal Protective Equipment【個人防護裝備】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
